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In vitro anti-collagenase activity of Sri Lankan low grown orthodox Orange Pekoe grade black tea (Camellia sinensis L.)

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Author Affiliations

  • 1Faculty of Allied Health Sciences, General Sir John Kotelawala Defence University, Ratmalana, Sri Lanka and Department of Zoology, University of Colombo, Colombo-03, Sri Lanka
  • 2Herbal Technology Section, Industrial Technology Institute (ITI), 363, Bauddhaloka Mawatha, Colombo 07, Sri Lanka
  • 3Herbal Technology Section, Industrial Technology Institute (ITI), 363, Bauddhaloka Mawatha, Colombo 07, Sri Lanka
  • 4Faculty of Medicine, University of Colombo, Colombo-08, Sri Lanka
  • 5Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka

Res.J.chem.sci., Volume 7, Issue (7), Pages 11-15, July,18 (2017)

Abstract

Anti-collagenase activity of tea brew of Sri Lankan low grown orthodox Orange Pekoe grade black tea (Camellia sinensis L.) was evaluated using five concentrations (25, 50, 75, 100 and 200 µg/ml) of black tea brew (BTB) made according to ISO specifications. Anti-collagenase activity of BTB and tea catechin, epigallocatechingallate (EGCG) were ascertained in vitro using collagenase enzyme from Clostridium histolyticum and a synthetic substrate (FALGPA) using spectroscopy. BTB induced marked and significant (P < 0.05) anti-collagenase activity (IC50 = 80.04 ± 2.34 µg/ml). This effect was dose dependent. Moreover, anti-collagenase activity of BTB was superior to EGCG (IC50 = 112.12 ± 0.93 µg/ml), a well known anti-collagenase phytoconstituent of green tea. The results convincingly show that Sri Lankan low grown orthodox Orange Pekoe grade black tea possesses remarkable anti-collagenase activity in vitro and display its promise to be developed as a potent anti-aging skin nutraceutical.

References

  1. Baumann L. (2007)., Skin ageing and its treatment., J Pathol., 211, 241-251.
  2. Mukherjee P.K., Maity N., Nema N.K. and Sarkar B.K. (2011)., Bioactive compounds from natural resources against skin ageing., Phytomedicine., 19(1), 64-73.
  3. Young B., Lowe J.S., Stevens A. and Heath J.W. (2002)., Wheater’s Functional Histology: A Text and Colour Atlas., 4th ed. New Delhi: Reed Elsevier India Private Limited, 167-187.
  4. Thring T.S., Hill P. and Naughton D.P. (2009)., Anti-collagenase, anti-elastase and antioxidant activities of extracts from 21 plants., BMC Complement Altern Med., 9, 27-38.
  5. Nomita P., Mukesh R. and Vijay K.J. (2012)., Camellia sinensis (Green Tea): A review., Global J Pharmacol., 6, 52-59.
  6. Lee K.K., Kim J.H., Cho J.J. and Choi J.D. (1999)., Inhibitory effects of 150 plant extracts on elastase activity, and their anti-inflammatory effects., Int J Cosmet Sci., 21(2), 71-82.
  7. Ratnasooriya W.D., Abeysekera W.P.K.M. and Muthunayake T.B.S. (2013)., Effect of Sri Lankan low grown orthodox black tea (Camellia sinensis L.) on in vitro anti-elastase activity., Int J Res Pharm Biomed Sci., 4, 1034-1037.
  8. Sri Lanka Tea Board Annual Report (2011)., Sri Lanka Tea Board, Colombo, Sri Lanka., 6-21.
  9. International Organization for Standardization (1980).Tea-Determination of loss of mass at 103 xC (Moisture), ISO 1573, Geneva, Switzerland, 1-4., undefined, undefined
  10. International Organization for Standardization (1980). Tea-Determination of total ash, ISO 1575, Geneva, Switzerland, 1-3., undefined, undefined
  11. International Organization for Standardization (1988). Tea-Determination of water soluble ash, ISO 1576, Geneva, Switzerland, 1-2., undefined, undefined
  12. International Organization for Standardization (1987). Tea-Determination of acid-insoluble ash, ISO 1577, Geneva, Switzerland, 1-2., undefined, undefined
  13. Atmosphere S. (1975)., International Organization for Standardization., Tea-Determination of alkalinity of water soluble ash, ISO 1578, Geneva, Switzerland, 1-2.
  14. International Organization for Standardization (1978)., Animal and Vegetable Fats and Oils: Preparation of Methyl Esters of Fatty Acids., Tea-Determination of loss of water extract. ISO 9768. Geneva, Switzerland, 1-3.
  15. International Organization for Standardization (1999). Tea-Determination of crude fibre content. ISO 15198, Geneva, Switzerland, 1-5., undefined, undefined
  16. Test method for substances characteristic of green and black tea. (2005)., Part 1: Determination of total polyphenols in tea, colourimetric method using Folin-clocateu reagent., ISO /DIS 14502-1, ISO Technical Programme, TC 34ISC8, pp 1-4.
  17. ISO (2005)., 14502-2 Determination of substances characteristic of green and black tea—Part2: Content of catechins in green tea—Method using high-performance liquid chromatography., ISO/DIS 14502-2, ISO Technical Programme, TC /34/SC.8, 5-7.
  18. Geneva S. (1980)., International Organization for Standardization., Tea preparation of liquor for use in sensory tests, ISO 3103, Geneva, Switzerland, 1-4.
  19. Van wart H.E. and Steinbrink D.R. (1981)., A continuous spectrophotometric assay for Clostridium histolyticum collagenease., Anal Biochem., 113(2), 356-365.
  20. Ratnasooriya W.D. (2012)., An assessment of potential health benefits of Sri Lankan black tea by studying its bioactivities II., 1st six month Report, National Science Foundation of Sri Lanka, Colombo, Sri Lanka. (Grant No: NSF/Fellow/2011/01).
  21. Barrantes E. and Guinea M. (2003)., Inhibition of collagenase and metalloproteinase by aloins and aloe gel., Life Sci., 72(7), 843-850.
  22. Modder W.W.D. and Amarakoon A.M.T. (2002)., Tea and Health., 1st ed. Tea Research Institute: Thalawakelle: Sri Lanka, 1-179.
  23. Ratnasooriya W.D., Jayakody J.R.A.C., Rosa S.R.D. and Ratnasooriya C.D.T. (2014)., In vitro sun screening activity of Sri Lankan orthodox black tea (Camellia sinensis L.)., World J Pharm Sci., 2, 144-148.
  24. Ratnasooriya W.D., Abeysekera W.K.S.M., Muthunayake T.B.S. and Ratnasooriya C.D.T. (2014)., In vitro antiglycation and cross-link breaking activities of Sri Lankan low grown orthodox Orange Pekoe grade black tea (Camellia sinensis L.)., Trop J Pharm Res., 13, 567-571.
  25. Ratnasooriya W.D., Abeysekera W.P.K.M. and Ratnasooriya C.D.T. (2014)., In vitro skin whitening and lightening properties of Sri Lankan orthodox Orange Pekoe grade black tea (Camellia sinensis L.)., World J Pharm Sci., 2, 1249-1252.
  26. Ratnasooriya W.D., Abeysekera W.P.K.M. and Ratnasooriya C.D.T. (2014)., In vitro anti-hyaluronidase activity of Sri Lankan orthodox Orange Pekoe grade black tea (Camellia sinensis L.)., Asian Pac J Trop Biomed., 4(12), 959-963.
  27. Ratnasooriya W.D. (2008)., An assessment of potential health benefits of Sri Lankan black tea by studying its bioactivities., I. Final Report, National Science Foundation of Sri Lanka, Colombo, Sri Lanka, (Grant No: NSF/Fellow/2005/01), 85-105.
  28. Hori M., Yagi M., Nomoto K., Shimode A., Ogura M. and Yonei Y. (2012)., Inhibition of advanced glycation end product formation by herbal teas and its relation to anti-skin ageing., Anti-ageing Medicine., 9(6), 135-148.
  29. Ratnasooriya W.D. and Fernando T.S.P. (2009)., Anti-inflammatory activity of Sri Lankan black tea (Camellia sinensis) in rats., Pharmacogn Res., 1, 11-20.